polyclonal sheep  (R&D Systems)

Journal: Archives of histology and cytology

Article Title: Neurocan contributes to the molecular heterogeneity of the perinodal ECM.

doi: 10.1679/aohc.73.95

Figure Lengend Snippet: Fig. 1. A Western blot analysis of an affinity- purified anti-neurocan antibody. Adult brain or optic nerve lysates (brain, 30μg; optic nerve, 15μg) treated with Ch'ase ABC were resolved in 2–15% gradient SDS-PAGE gels and immunoblotted with an affiniy-purified anti- neurocan antibody, anti-brevican antibody, and anti-versican-α antibody. Notice the specific bands for the full length neurocan (Ncan full) and proteolytic fragments (Ncan190, Ncan150, and Ncan90). Also notice the absence of cross- reacted bands for link proteins at around 35–45 kDa (asterisk). Ncan: neurocan, Bcan: brevican, V2: versican V2, on: optic nerve.

Article Snippet: We used a rabbit anti-caspr polyclonal antibody ab34151: Abcam, Cambridge, MA, USA; 1 μg/ml for immunohistchemistry) and a sheep antimouse/rat neurocan polyclonal antibody (AF5800: R&D systems, Minneapolis, MN, USA; 8 μg/ml for immunohistchemistry and 2 μg/ml for immunoblotting) for our studies.

Techniques: Western Blot, Affinity Purification, SDS Page

Journal: Archives of histology and cytology

Article Title: Neurocan contributes to the molecular heterogeneity of the perinodal ECM.

doi: 10.1679/aohc.73.95

Figure Lengend Snippet: Fig. 3. Immunohistochemical staining of neurocan in the white matter region and the nerve fiber tracts of the brain. The dotted lines indicate the border of the white matter region. Punctate patterns of neurocan-positive staining are observed in the corpus callosum (A, cc), the internal capsule (B, ic) and the zona incerta (B, ZI), the optic nerve (C, on), the facial nerve tract (D, 7n) and the superior olive (D, SO). Bar: 50μm

Article Snippet: We used a rabbit anti-caspr polyclonal antibody ab34151: Abcam, Cambridge, MA, USA; 1 μg/ml for immunohistchemistry) and a sheep antimouse/rat neurocan polyclonal antibody (AF5800: R&D systems, Minneapolis, MN, USA; 8 μg/ml for immunohistchemistry and 2 μg/ml for immunoblotting) for our studies.

Techniques: Immunohistochemical staining, Staining

Journal: Archives of histology and cytology

Article Title: Neurocan contributes to the molecular heterogeneity of the perinodal ECM.

doi: 10.1679/aohc.73.95

Figure Lengend Snippet: Fig. 2. Immunohistochemical staining of cerebellar sections from adult mice. Cerbellar sections were stained using antibodies against neurocan. (A), brevican (B), or Bral1 (C). Notice the presence of the punctuate-immunoreactivity in the white matter with a concomitant perineuronal net type-immunoreactivity in the deep cerebellar nuclei (DC) with the anti-neurocan antibody. Ncan: neurocan, Bcan: brevican. Bar: 100μm

Article Snippet: We used a rabbit anti-caspr polyclonal antibody ab34151: Abcam, Cambridge, MA, USA; 1 μg/ml for immunohistchemistry) and a sheep antimouse/rat neurocan polyclonal antibody (AF5800: R&D systems, Minneapolis, MN, USA; 8 μg/ml for immunohistchemistry and 2 μg/ml for immunoblotting) for our studies.

Techniques: Immunohistochemical staining, Staining

Journal: Archives of histology and cytology

Article Title: Neurocan contributes to the molecular heterogeneity of the perinodal ECM.

doi: 10.1679/aohc.73.95

Figure Lengend Snippet: Fig. 4. Immunofluorescent labeling of the optic nerve in wild-type mice (A, C–E) and Bral1-deficient mice (B, F–H) using antibodies to neurocan (magenta), Bral1 (green), and caspr (green). A neurocan signal is clearly shown between caspr-positive paranodes (A). Punctate signals of neurocan are accompanied with Bral1 (E, arrows). Notice the presence of some neurocan- negative nodes (arrow heads). Ncan: neurocan, Bcan: brevican. Bars: 10μm

Article Snippet: We used a rabbit anti-caspr polyclonal antibody ab34151: Abcam, Cambridge, MA, USA; 1 μg/ml for immunohistchemistry) and a sheep antimouse/rat neurocan polyclonal antibody (AF5800: R&D systems, Minneapolis, MN, USA; 8 μg/ml for immunohistchemistry and 2 μg/ml for immunoblotting) for our studies.

Techniques: Labeling

Journal: Archives of histology and cytology

Article Title: Neurocan contributes to the molecular heterogeneity of the perinodal ECM.

doi: 10.1679/aohc.73.95

Figure Lengend Snippet: Fig. 5. Double immunostaining for neurocan and brevican in the optic nerve. Notice the presence of brevican-positive nodes (arrows) and brevican-negative nodes (arrow heads) among the neurocan-positive nodes. Ncan: neurocan, Bcan: brevican. Bar: 10μm

Article Snippet: We used a rabbit anti-caspr polyclonal antibody ab34151: Abcam, Cambridge, MA, USA; 1 μg/ml for immunohistchemistry) and a sheep antimouse/rat neurocan polyclonal antibody (AF5800: R&D systems, Minneapolis, MN, USA; 8 μg/ml for immunohistchemistry and 2 μg/ml for immunoblotting) for our studies.

Techniques: Double Immunostaining